mapping and expression analysis of a fusarium head blight resistance gene candidate pleiotropic drug resistance 5 (pdr5) in wheat
نویسندگان
چکیده
fusarium head blight (fhb) caused by fusarium graminearum is a serious disease of wheat (triticum aestivum l.), through which grain quality losses are induced by fungal trichotecene mycotoxins such as deoxynivalenol (don). a class of plasma membrane localized abc transporter proteins related to the yeast pdr5 (pleiotropic drug resistance5) efflux pump seems to be responsible for partial resistance against trichothecenes in wheat. in order to develop and map a pdr5-specific marker linked to fusarium head blight resistance in wheat, f3 and f5 generations obtained from a cross between ‘wangshuibai’ and ‘seri82’ were used. the analysis of nucleotide sequences of ospdr5 revealed a high homology to the wheat est bt009500. among ten primer pairs developed from this pdr5-like est, one was polymorphic between the parental lines. this pcr-based marker associated with fhb resistance in a ‘wangshuibai’ derived mapping population. in this study, composite interval mapping (cim) analysis detected a qtl in the map interval of xm12p17_2-xpdr5 (consisting of the developed pdr5-like gene locus) on chromosome 6bs. this qtl accounted for up to 18% of audpc variation. real-time quantitative analysis showed that wheat pdr5-like gene expression was up-regulated during the post-inoculation period of 96 hours in the spike. our results are in agreement with the hypothesis that the pdr5-like gene may be considered as a fhb resistance gene candidate in wheat.
منابع مشابه
Mapping and Expression Analysis of a Fusarium Head Blight Resistance Gene Candidate Pleiotropic Drug Resistance 5 (PDR5) in Wheat
Fusarium head blight (FHB) caused by Fusarium graminearum is a serious disease of wheat (Triticum aestivum L.), through which grain quality losses are induced by fungal trichotecene mycotoxins such as deoxynivalenol (DON). A class of plasma membrane localized ABC transporter proteins related to the yeast PDR5 (pleiotropic drug resistance5) efflux pump seems to be responsible for partial resista...
متن کاملIdentification and characterization of a fusarium head blight resistance gene TaACT in wheat QTL‐2DL
Fusarium head blight (FHB) resistance in wheat is considered to be polygenic in nature. Cell wall fortification is one of the best resistance mechanisms in wheat against Fusarium graminearum which causes FHB. Metabolomics approach in our study led to the identification of a wide array of resistance-related (RR) metabolites, among which hydroxycinnamic acid amides (HCAAs), such as coumaroylagmat...
متن کاملIntegrated Metabolo-Transcriptomics Reveals Fusarium Head Blight Candidate Resistance Genes in Wheat QTL-Fhb2
BACKGROUND Fusarium head blight (FHB) caused by Fusarium graminearum not only causes severe losses in yield, but also reduces quality of wheat grain by accumulating mycotoxins. Breeding for host plant resistance is considered as the best strategy to manage FHB. Resistance in wheat to FHB is quantitative in nature, involving cumulative effects of many genes governing resistance. The poor underst...
متن کاملGenome-Wide Association Mapping of Fusarium Head Blight Resistance in Wheat using Genotyping-by-Sequencing.
Fusarium head blight (FHB) is one of the most important wheat ( L.) diseases worldwide, and host resistance displays complex genetic control. A genome-wide association study (GWAS) was performed on 273 winter wheat breeding lines from the midwestern and eastern regions of the United States to identify chromosomal regions associated with FHB resistance. Genotyping-by-sequencing (GBS) was used to...
متن کاملWhole Genome Association Mapping of Fusarium Head Blight Resistance in European Winter Wheat (Triticum aestivum L.)
A total of 358 recent European winter wheat varieties plus 14 spring wheat varieties were evaluated for resistance to Fusarium head blight (FHB) caused by Fusarium graminearum and Fusarium culmorum in four separate environments. The FHB scores based on FHB incidence (Type I resistance)×FHB severity (Type II resistance) indicated a wide phenotypic variation of the varieties with BLUE (best linea...
متن کاملMapping QTLs for Fusarium Head Blight Resistance in an Interspecific Wheat Population
Fusarium head blight (scab) is one of the most widespread and damaging diseases of wheat, causing grain yield and quality losses and production of harmful mycotoxins. Development of resistant varieties is hampered by lack of effective resistance sources in the tetraploid wheat primary gene pool. Here we dissected the genetic basis of resistance in a new durum wheat (Triticum turgidum ssp. durum...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of biotechnologyناشر: national institute of genetic engineering and biotechnology
ISSN 1728-3043
دوره 8
شماره 2 2010
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023